IDENTIFICATION OF BIOLOGICAL MATERIALS OF CATTLE USING GENETIC MARKERS

Abstrak

Genetic material (DNA) related to cattle is extracted from biological samples provided for research. These samples may include meat samples, skin samples, hair, horns, and bone samples. Specific requirements are set for pieces of animals' muscle or other tissues. The methods of DNA extraction from different biological materials of cattle vary by method. Аfter extraction the concentration of extracted DNA was quantified by using the Qubit 4.0 fluorometer (Thermo Fisher Scientific, USA) and the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, USA), following the manufacturer's recommended protocols. For the PCR amplification process of the extracted cattle DNA samples, the "COrDIS Cattle" kit was used. The PCR amplification process was performed using the COrDIS Cattle kit protocol. The following amplification requirements are recommended as standard parameters. It is important to control the heating rate during the temperature rise from 59°C to 72°C to be 0.3°C per second. Due to the high complexity of the amplification involving 15 primer pairs, this heating rate is crucial for the optimal efficiency of the reaction.