TABIIY HOM ASHYOLAR TARKIBIDAN LEKTIN MODDASINI AJRATIB OLISH VA UNING FIZIK-KIMYOVIY XUSUSIYATLARINI O’RGANISH
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Lektin, Phaseolus vulgaris, oqsillarni tozalash, Affi-gel blue gel, FPLC (Tez oqsillar suyuqli xromatografiyasi), ion almash xromatografiyasi, gel filtratsiyasi, subbirliklar, N-terminal tuzilishi, gemagglutinatsiya faolligi, pH va harorat barqarorligi, MCF-7 hujayralari, ko‘krak bezi saratoni, HIV-1 teskari transkriptaza, ingibitsiya, mitogenik javob, timidin singishi, makrofaglar, azot oksidi hosil bo‘lishi, kimyoviy modifikatsiya, triptofan va faollik.Abstrak
Lektin Phaseolus vulgaris urug'idan ajratilgan bo'lib, u affi-gel blue gel, tez oqsillar suyuqli xromatografiyasi (FPLC)-ion almash xromatografiyasi va FPLC-gel filtratsiyasi kabi usullar yordamida tozalangan. Lektin ikkita 30 kDa subbirliklardan tashkil topgan bo'lib, ularning N-terminal tuzilishi boshqa Phaseolus lektinlariga o'xshashligi aniqlangan. Lektinning gemagglutinatsiya faolligi pH 1-14 va harorat 0-80°C oralig'ida barqarorligicha qoldi. Lektin MCF-7 (ko'krak bezi saratoni) hujayralarining proliferatsiyasini IC50 1.3 μM bilan kuchli bostirib tashladi va HIV-1 teskari transkriptazasining faolligini IC50 7.6 μM bilan ingibitsiya qildi. Lektin, shuningdek, sichqon splenotsitlarida mitogenik javobni keltirib chiqarish orqali [3H-metil]-timidinning singishini oshirish orqali o'z faolligini namoyon qildi. Lektin murin peritoneal makrofaglarida azot oksidi hosil qilishini rag'batlantirmadi. Kimyoviy modifikatsiya natijalari shuni ko'rsatdiki, triptofan lektinning gemagglutinatsiya faolligiga juda muhimdir.
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