STUDY OF THE ANTI-ULCER ACTIVITY OF A PHYTOCOMPETENT WITH ADAPTOGENIC PROPERTIES
Keywords:
Salvia Sclarea L., Matricaria Chamomilla L., tannin, acute toxicity, LD₅₀, Rainier method, Bulbring and Vida method, anesthetic activity.Abstract
In this article, the acute toxicological properties and local analgesic (anesthetic) activity of tannins isolated from plants Salvia Sclarea L. (sage) and Matricaria Chamomilla L. (chamomile) were studied on an experimental animal model (mice). The acute toxicity index was studied by oral administration (per os) of the substance to animals in various doses (100.0–15800.0 mg/kg). The experimental results showed that the acute toxicity index, estimated by LD₅₀, was 14600.3 mg/kg with oral administration of tannin, which confirms the classification of this substance as low-toxic. The anesthetic activity was studied using the methods of Rainier, as well as Bulbring and Vida. In experiments conducted using the Rainier method with 0.5% tannin solution, the time of onset of anesthesia was 3.7±0.3 minutes, the time of complete anesthesia was 12.1±0.7 minutes, and the total duration was 15.8 ± 0.9 minutes. The results of the Bulbring and Vida method confirmed that the substance has a dose-dependent pain-reducing effect; in this model, the maximum effect of 0.5% tannin solution was also observed. The results of the study show the prospects of introducing tannins of natural origin into medical practice as a low-toxic compound with local analgesic effect.
References
Бобылев А. Г. Местные анестетики: история, свойства и применение // Российский медицинский журнал. – 2017. – Т. 23, №4.– С. 18–22.
Юмашев А. В., Фахрутдинов Р. Г. Современные подходы к разработке природных местных анестетиков // Вестник новых медицинских технологий.– 2020. – Т. 27, №1. – С. 53–57.
Becker D.E., Reed K.L. Essentials of Local Anesthetic Pharmacology // Anesthesia Progress. – 2012. – Vol. 59(2). – P. 90–101.DOI: 10.2344/0003-3006-59.2.90
Chazotte C. Natural Products as Sources of New Drugs over the 30 Years from 1981 to 2010 // Journal of Natural Products.– 2012. – Vol. 75(3). – P. 311–335. DOI: 10.1021/np200906s
Gasparotto J., Somensi N., Reus G.Z. et al. Anti-nociceptive effects of Matricaria Chamomilla L. extract in animal models // Phytomedicine.– 2018. – Vol. 39. – P. 1–5. DOI: 10.1016/j.phymed.2017.12.011
ГОСТ Р 53434–2009. Лабораторные животные. Правила содержания и ухода. Москва: Стандартинформ, 2009.
ГОСТ ISO/IEC 17025–2019. Общие требования к компетентности испытательных и калибровочных лабораторий. Москва: Стандартинформ, 2019.
OECD Guidelines for the Testing of Chemicals. Acute Oral Toxicity – Acute Toxic Class Method. OECD Publishing, 2017.
Шамсиев А.Х. Биологически активные соединения лекарственных растений. – Т.: Фан, 2016. – 248 с.
Кузнецов С.Л. Методы оценки токсичности лекарственных средств. – М.: ГЭОТАР-Медиа, 2018. – 296 с.
Gasparotto J. et al. Anti-nociceptive and anti-inflammatory properties of Chamomilla recutita extract in mice. // Phytomedicine. – 2018. – Vol.39. – P. 1–5. DOI: 10.1016/j.phymed.2017.12.011
Gintzler A.R., Liu N.J. Renie’s corneal reflex as a model for evaluating peripheral anesthetics // Current Protocols in Pharmacology.– 2018. – Vol. 82. – P. 12–16.
Миронов А.Н. Руководство по проведению доклинических исследований лекарственных средств. Часть первая. М: Гриф и К, 2012.-944 с.
Ahmed M.M. et al. Evaluation of local anesthetic activity using Bulbring–Wajda method in guinea pigs // Journal of Experimental Pharmacology.
– 2015. – Vol. 7. – P. 43–49.
Стефанова А.В. «Доклинические исследования лекарственных средств», Киев 2002, 528 с.
Руководство по экспериментальному (доклиническому) изучению новых фармакологических веществ / под общ. ред. Р.У. Хабриева. – 2-е изд., перераб. и доп.– М.: ОАО «Издательство «Медицина», 2005. 832 с.
Klinikagacha bo'lgan farmakologik izlanish asoslari: darslik / R.T. Tulyaganov, Z.T. Fayziyeva, R.X. Sultanova, A.N. Abdixakimov.-Toshkent: Invest book , 2024. 220 b.
Бережной В.В., Калинина Е.А. Биостатистика в Microsoft Excel: практическое руководство для медиков и биологов.– М.: ГЭОТАР-Медиа, 2021. – 192 с. ISBN: 978-5-9704-6215-3
