“THE ROLE OF AGAROSE GEL ELECTROPHORESIS IN THE VISUALIZATION OF PCR PRODUCTS IN MOLECULAR BIOLOGY"
Keywords:
Gel, electrophoresis, DNA, molecular biology, genetics, standard.Abstract
This study investigates the importance of standard agarose gel electrophoresis in the detection of PCR (polymerase chain reaction) products. The process of separation and visualization of DNA fragments obtained through PCR was analyzed. Agarose gel electrophoresis allows effective separation of DNA fragments based on their size. The results demonstrate that this method is simple, reliable, and widely used for detecting PCR products. This approach has significant practical importance in the fields of molecular biology and genetics.
References
Mullis, K., & Faloona, F. (1987). Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods in Enzymology, 155, 335–350.
Sambrook, J., & Russell, D. W. (2001). Molecular Cloning: A Laboratory Manual (3rd ed.). Cold Spring Harbor Laboratory Press.
Brown, T. A. (2016). Gene Cloning and DNA Analysis: An Introduction (7th ed.). Wiley-Blackwell.
Green, M. R., & Sambrook, J. (2012). Molecular Cloning: A Laboratory Manual (4th ed.). Cold Spring Harbor Laboratory Press.
Alberts, B., Johnson, A., Lewis, J., et al. (2015). Molecular Biology of the Cell (6th ed.). Garland Science.
Watson, J. D., Baker, T. A., Bell, S. P., et al. (2014). Molecular Biology of the Gene (7th ed.). Pearson.
Lee, P. Y., Costumbrado, J., Hsu, C. Y., & Kim, Y. H. (2012). Agarose gel electrophoresis for the separation of DNA fragments. Journal of Visualized Experiments, (62), e3923.
Lorenz, T. C. (2012). Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies. Journal of Visualized Experiments, (63), e3998.
