"MOLEKULYAR BIOLOGIYADA PCR MAHSULOTLARINI VIZUALIZATSIYA QILISHDA AGAROZ-GEL ELEKTROFOREZINING ROLI"
Ключевые слова:
Gel, elektroforez, DNK, molekulyar biologiya, genetika, standart.Аннотация
Ushbu ishda PCR (polimeraza zanjir reaksiyasi) mahsulotlarini aniqlashda standart agaroz gel elektroforez usulining ahamiyati o‘rganildi. Tadqiqot davomida PCR orqali olingan DNK fragmentlarini ajratish va vizualizatsiya qilish jarayoni tahlil qilindi. Agaroz gel elektroforez usuli DNK fragmentlarini ularning uzunligiga qarab samarali ajratish imkonini berishi ko‘rsatildi. Natijalar shuni ko‘rsatdiki, mazkur usul PCR mahsulotlarini aniqlashda oddiy, ishonchli va keng qo‘llaniladigan metod hisoblanadi. Ushbu yondashuv molekulyar biologiya va genetika sohasida muhim amaliy ahamiyatga ega.
Библиографические ссылки
Mullis, K., & Faloona, F. (1987). Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods in Enzymology, 155, 335–350.
Sambrook, J., & Russell, D. W. (2001). Molecular Cloning: A Laboratory Manual (3rd ed.). Cold Spring Harbor Laboratory Press.
Brown, T. A. (2016). Gene Cloning and DNA Analysis: An Introduction (7th ed.). Wiley-Blackwell.
Green, M. R., & Sambrook, J. (2012). Molecular Cloning: A Laboratory Manual (4th ed.). Cold Spring Harbor Laboratory Press.
Alberts, B., Johnson, A., Lewis, J., et al. (2015). Molecular Biology of the Cell (6th ed.). Garland Science.
Watson, J. D., Baker, T. A., Bell, S. P., et al. (2014). Molecular Biology of the Gene (7th ed.). Pearson.
Lee, P. Y., Costumbrado, J., Hsu, C. Y., & Kim, Y. H. (2012). Agarose gel electrophoresis for the separation of DNA fragments. Journal of Visualized Experiments, (62), e3923.
Lorenz, T. C. (2012). Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies. Journal of Visualized Experiments, (63), e3998.
