Word order plays a crucial role in sentence structure, meaning, and clarity in any language. English and Uzbek differ significantly in this aspect: English and Uzbek differ significantly in word order: English follows SVO, while Uzbek primarily uses SOV. This difference influences sentence formation and emphasis. These differences affect emphasis, sentence interpretation, and overall communication strategies. In English, altering word order can lead to ungrammatical sentences or change the intended meaning. In contrast, Uzbek allows for greater flexibility, enabling speakers to shift emphasis without losing grammatical correctness. This paper explores the fundamental differences between English and Uzbek word order, examining their impact on sentence formation, emphasis, and question structures. Understanding these distinctions is essential for linguists, translators, and language learners seeking to master both languages.